Composite

Part:BBa_K4229049:Design

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-29)


"Full wiffelball" with tags, under regulation of lambdapLhybrid promotor and LacI promotor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 444
    Illegal PstI site found at 1711
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 444
    Illegal PstI site found at 1711
    Illegal NotI site found at 1515
    Illegal NotI site found at 2892
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 444
    Illegal BglII site found at 453
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 444
    Illegal PstI site found at 1711
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 444
    Illegal PstI site found at 1711
    Illegal NgoMIV site found at 335
    Illegal NgoMIV site found at 842
    Illegal NgoMIV site found at 2512
    Illegal NgoMIV site found at 2868
    Illegal AgeI site found at 1319
    Illegal AgeI site found at 1427
    Illegal AgeI site found at 2014
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

The plasmid containing this protein was kindly sent to us by the Kerfeld group.

References

H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, DOI: 10.1186/s12915-019-0691-z.

H. Kirst, B. H. Ferlez, S. N. Lindner, C. A. R. Cotton, A. Bar-Even, and C. A. Kerfeld, “Toward a glycyl radical enzyme containing synthetic bacterial microcompartment to produce pyruvate from formate and acetate,” Proc. Natl. Acad. Sci. U. S. A., vol. 119, no. 8, pp. 1–10, 2022, doi: 10.1073/pnas.2116871119.